Separation and identification of flavonoid constituent in Humulus Scandens and their effects on alveolar fluid clearance in mice

VernacularTitle:葎草中黄酮类成分的分离鉴定及对小鼠肺水清除率的影响
Author: Zaixing CHEN ; Xing WANG ; Yan ZHU ; Lin WANG ; Yan WANG ; Hongguang NIE ; Fanhao MENG
Publication Type:Journal Article
Keywords: Humulus Scandens; chemical constituent; luteolin-7-O-β-D-glucoside; cosmsiin; alveolar fluid clearance (AFC); pulmonary edema
From: Chinese Pharmacological Bulletin 2015;(12):1715-1718
CountryChina
Language:Chinese
Abstract: Aims To extract,separate and identify the flavonoid constituents in Humulus Scandens and to ex-plore the relationship of monomers and alveolar fluid clearance (AFC)in mice in vivo.Methods Humulus scandens were extracted with alcohol and then isolated by the technology of Column and the structures were i-dentified by spectrometry.In vivo AFC was measured using bovine serum albumin protein assays affected by luteolin-7-O-β-D-glucoside (LGL ) and cosmsiin (AGL).Results The main constituents of flavanones
in Humulus scandens were LGL and AGL.Both of them could improve the AFC.Conclusion The AFCs of LGL and AGL,compared to the blank control group, increased which explains the effect of flavonoid constit-uents on removing edema and promoting water absorp-tion.