Potent rapid antidepressant effects of petroleum ether fraction of Yueju pill associated with BDNF and TrkB up-regulation
10.3969/j.issn.1001-1978.2015.12.025
- VernacularTitle:越鞠丸石油醚部位潜在的快速抗抑郁作用与BDNF、TrkB 蛋白表达的上调相关
- Author:
Li REN
;
Weiwei TAO
;
Wenda XUE
;
Haoxin WU
;
Gang CHEN
- Publication Type:Journal Article
- Keywords:
Yueju pill;
petroleum ether fraction;
rap-id antidepressant;
BDNF;
TrkB;
ketamine
- From:
Chinese Pharmacological Bulletin
2015;(12):1754-1759
- CountryChina
- Language:Chinese
-
Abstract:
Abstact:Aim To identify whether the petroleum e-ther fraction of Yueju pill (YJ-PE)could exhibit a po-tent rapid antidepressant effect and to investigate the underlying biological mechanism involved.Methods Fourty-four male KM mice were randomly assigned to five groups:the control group (saline group),low dosage of YJ-PE,middle dosage of YJ-PE,high dos-age of YJ-PE and ketamine.After a single drug admin-istration,we used tail suspension test (TST)to test the rapid antidepressant effect of YJ-PE in KMmice at 30 min.Another twenty-four male KM mice were ass-inged to three groups:control group,screened effective low dosage of YJ-PE group and ketamine group.We usd TST to assess these mice at 24 h post a single ad-
ministration.Western blot was performed to examine the expression of BDNF and TrkB in hippocampus of KMmice at 30 min and 24 h post a single administra-tion.Results An acute administration of YJ-PE de-creased the immobility time of KMmice in TST and in-creased the expression of BDNF and TrkB as soon as 30min post a single administration and lasted at least for 24 hours.Conclusion YJ-PE (low dosage)ex-hibits a rapid antidepressant-like potent,and the po-tent rapid antidepressant effects of YJ-PE are associat-ed with the up-regulation of BDNF and TrkB.
