Establishment and characteristic analysis of fibroblast-like synoviocytes in rats with adjuvant arthritis
10.3969/j.issn.1001-1978.2015.12.013
- VernacularTitle:佐剂型关节炎大鼠滑膜成纤维细胞模型建立及特征分析
- Author:
Wanjiao GAO
;
Qiudi DENG
;
Shutong BAI
;
Li TONG
- Publication Type:Journal Article
- Keywords:
fibroblast-like synoviocytes;
rheumatoid arthritis;
adjuvant arthritis rat;
Bcl-2;
Bax;
Cleave-caspase-3
- From:
Chinese Pharmacological Bulletin
2015;(12):1693-1698
- CountryChina
- Language:Chinese
-
Abstract:
Aims To establish the methods of primary culture of fibroblast-like synoviocytes in rats with adju-vant arthritis(AA-FLS)and analyze the feature and to investigate the possibility of AA-FLS as the model for the RA in vitro.Methods The synovial cells obtained from the SD rats were immunized by the Mtb and iden-tified by morphology and immunocytochemistry.The viability of AA-FLS was assessed by Cell Counting Kit-8.ELISA was applied to detect TNF-αand IL-1 βin cell media. Apoptosis was measured by Hochest 33258.The expressions of mitochondrial apoptosis-re-lated molecules,including Bcl-2,Bax,Pro-caspase-3 and Cleave-caspase-3 were determined by Western Blot.Result In isolated primary synovial cells,more
than 95% of AA-FLSs were fusiform.Immunocyto-chemistry result showed a positive expression of vimen-tin and a negative expression of CD68 in AA-FLS.Cell proliferation of AA-FLS was higher than FLS and cell apoptosis of AA-FLS was curbed.Western blot data demonstrated that the protein expressions of Bcl-2,Bax were regulated and the expression of caspase-3 was ac-tivated in AA-FLS.Conclusions AA-FLS is biologi-cally characterized by high level proliferation activity and inflammatory cytokines and apoptosis suppression. AA-FLS can be used as the model for the RA in vitro.
