Protective effect of Shenfu soup on myocardial ischemia in rats and expression of apelin
10.3969/j.issn.1001-1978.2015.12.026
- VernacularTitle:参附汤抗大鼠心肌缺血作用与 apelin 表达的相关性研究
- Author:
Zhi LIU
;
Jianquan WANG
;
Xingyun WANG
;
Chang LIU
;
Lixue SHEN
;
Huiying XU
;
Dafang ZHANG
- Publication Type:Journal Article
- Keywords:
Shenfu soup;
ISO;
myocardial ischemia;
CK;
LDH;
apelin
- From:
Chinese Pharmacological Bulletin
2015;(12):1760-1763
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effects of Shenfu soup onthe ISO -induced myocardial ischemia injury model ofrats and the influence of apelin level changes on myocardial ischemia.Methods The myocardial ischemiainjury model of rats was established by the subcutaneous injection of ISO.For 1 5 days,rats in Shenfugroups were given by gavage 3,6 and 1 2 g · kg -1Shenfu soup and rats in SF injection group were given6.67 g·kg -1 Shenfu injection,rats in sham group andmodel group were given the same volume of distilledwater.Rats were subcutaneously multipoint injectedISO for 5 days on the eleventh administrating day.Theeffects of Shenfu soup on myocardial morphology,serum myocardial enzyme levels,apelin mRNA level inserum and apelin protein level in myocardial tissue,and relationship between myocardial enzyme and apelinexpression were evaluated. Results Shenfu soupcould reduce the degree of myocardial tissue necrosisand compared with model group,CK values and LDHvalues of rats in middleand highdose groups were significantly lower (P <0.05 or P <0.01 ).However,the apelin mRNA level in myocardial tissue and apelinprotein level in serum of the rats in middleand highdose groups compared with model group had increased(P <0.05,P <0.01 ).Meanwhile,serum levels ofapelin and the expression of myocardial enzyme CK andLDH were negatively correlated.Conclusion Shenfusoup has a protective effect on myocardial ischemia injury in rats induced by ISO and the mechanism is involved with the promotion of apelin mRNA and proteinexpression,the inhibition of myocardial enzyme production and the improvement of myocardial ischemia.
