Serum miR-203 expression level detected by fluorescence quantitative PCR in cervical cancer patients and its significance
10.3969/j.issn.1673-4130.2015.24.018
- VernacularTitle:荧光定量 PC R检测宫颈癌患者血清中m iR-203表达水平及意义
- Author:
Chunlan YANG
;
Xianjuan SHEN
;
Shaoqin JU
;
Jianyou SU
- Publication Type:Journal Article
- Keywords:
SYBR Green Ⅰ real-time fluorescent quantitative PCR;
miR-203;
cervical cancer
- From:
International Journal of Laboratory Medicine
2015;(24):3552-3553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of the SYBR Green Ⅰ real‐time fluorescent quantitative PCR for detecting the se‐rum miR‐203 expression level ,and to detect the serum miR‐203 expression levels in the patients with cervical cancer ,cervical benign diseases and healthy controls .Methods The miR‐203 ,U6 stem loop RT primers and the PCR amplification primers were designed for conducting fluorescence quantitative PCR ,with U6 as the internal relative quantification ,the serum miR‐203 levels were com‐pared among different cervical diseases .Results The established method could specifically detect the amplification signal of serum miR‐203 ,the melting curve was single and PCR products were specific .The serum miR‐203 level in the patients with cervical cancer was significantly higher than that in the patients with benign cervical diseases such as hysteromyoma and cervicitis ,the difference was statistically significant (P<0 .05) .Conclusion The SYBR Green Ⅰ real‐time fluorescent quantitative PCR is a quick ,simple detection method with high sensitivity and good specificity ,which may have a better application prospect in cervical cancer auxiliary diagnosis .
