Application of DNA barcoding technique of COXⅠ gene in identification of ticks
10.3969/j.issn.1673-4130.2015.24.026
- VernacularTitle:COXⅠ基因DNA 条形码技术在蜱类鉴别中的应用
- Author:
Haiyan HOU
- Publication Type:Journal Article
- Keywords:
cytochrome C oxidase subunit Ⅰ gene;
DNA barcoding;
tick;
identification
- From:
International Journal of Laboratory Medicine
2015;(24):3570-3571
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of the cytochrome C oxidase subunit Ⅰ gene(COXⅠ ) barcoding in the i‐dentification of wild‐caught ticks .Methods The COXⅠ gene of 9 caught tick individuals with different species was sequenced ,the DNAstar software was used to conduct the sequence comparison and homology analysis .Meanwhile the philp3 .6 software was a‐dopted to select the maximal likelihood(ML) method for constructing the evolutionary trees .Results In the phylogenetic trees ,the samples of sh2011040702 ,ra2011042193 and ra2011051168 with Haemaphysalis were in a branch ,sh2011051404 ,sh2011051408 and ra2011041177 with dermacentor were clustered together ,and sh2011051837 ,sh2011041917 and ra2011041175 with hyalomma were in a branch ,which were consistent with the morphological identification results .Conclusion Making the COXⅠ gene sequence as tick DNA barcoding has certain feasibility for identifying the tick species .
