Identification of ATTM as a novel H2 S donor and investigation of its pro-tective effect on HaCaT skin cells
10.3969/j.issn.1000-4718.2015.12.027
- VernacularTitle:四硫代钼酸铵作为新型硫化氢供体的鉴定及其对皮肤细胞的保护作用
- Author:
Fuhui MENG
;
Li CHEN
;
Shi XU
;
Ming XIAN
;
Hui ZHANG
;
Jianhua LI
;
Qi DONG
;
Chuntao YANG
- Publication Type:Journal Article
- Keywords:
HaCaT cells;
Hydrogen sulfide;
Ammonium tetrathiomolybdate;
Oxidative stress
- From:
Chinese Journal of Pathophysiology
2015;(12):2271-2276
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the ability of a metal complex ammonium tetrathiomolybdate (ATTM) to re-lease H2 S and its cytoprotective effect on an oxidative injury model .METHODS:Released H2 S was absorbed in a reaction flask from ATTM dissolved in the cell medium .Staining with dichlorodihydrofluorescein diacetate or rhodamine 123 fol-lowed by photofluorography was conducted for the observation of reactive oxygen species ( ROS) and mitochondrial mem-brane potential (ΔΨm) levels, respectively.Cell viability and release of lactate dehydrogenase (LDH) from the cells were measured with commercial kits.RESULTS:Similar to another H2S donor GYY4137, ATTM had an ability to release H2S in the cell medium in a dose-dependent manner .Treatment of human skin HaCaT cells with ATTM at concentrations of 25~400 μmol/L didn’ t significantly alter cell viability .Exposure of the cells to ultraviolet rays or a ROS donor H 2 O2 in-creased the intracellular ROS levels .Treatment with 400 μmol/L H2 O2 significantly reduced the viability of HaCaT cells (P<0.01).However, before the treatment with H2O2, pretreatment with ATTM at 100 and 200 μmol/L markedly pre-vented the H2O2-induced cell injury (P<0.01).In addition, the treatment with H2O2 triggeredΔΨm loss (P<0.01) and LDH release from the cells (P<0.01).Prior to suffering from H2O2 injury, the preconditioning with 200 μmol/L ATTM significantly improved ΔΨm levels ( P<0.05 ) and attenuated LDH release from the cells ( P<0.01 ) .CONCLUSION:ATTM is capable of releasing H 2 S and protecting human skin cells against oxidative injury .
