Chemerin promotes proliferation of mouse vascular smooth muscle cells by up-regulating p-JNK
10.3969/j.issn.1000-4718.2015.12.029
- VernacularTitle:趋化素通过上调磷酸化 JNK 促进小鼠血管平滑肌细胞的增殖
- Author:
Wei XIONG
;
Shaohong DONG
;
Jian ZHANG
;
Jianghua LI
;
Bihong LIAO
;
Xinli PANG
;
Linjie LUO
- Publication Type:Journal Article
- Keywords:
Vascular smooth muscle cells;
Chemerin;
Cell proliferation;
c-Jun N-terminal kinase
- From:
Chinese Journal of Pathophysiology
2015;(12):2282-2286
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the proliferation property of stable chemerin gene knockdown vascular smooth muscle cells ( VSMCs) and to explore its mechanism .METHODS:The normal VSMCs , chemerin gene interfering control VSMCs and stable chemerin gene knockdown VSMCs were divided into normal group , PDGF group, control group and knockdown group .The VSMCs in PDGF group were given platelet-derived growth factor-BB ( PDGF-BB) to initiate proli-feration.The cell counting and BrdU assay were employed to investigate the proliferation property of VSMCs .The mitogen-activated protein kinase ( MAPK) signal pathway was determined by Western blot .RESULTS:The cell number and BrdU A value in PDGF-BB-treated VSMCs significantly increased as compared with the normal VSMCs ( P<0.05 ) .Compared with the normal VSMCs , the chemerin knockdown VSMCs showed obviously decreased cell number and BrdU A value ( P<0.05).Simultaneously, no significant difference in the proliferation of VSMCs between the normal VSMCs and the control VSMCs was observed.No significant difference of the protein levels of p-ERK1/2, ERK1/2, p-p38 MAPK and p38 MAPK among 4 kinds of VSMCs was found .The protein level of p-JNK in PDGF-BB-treated VSMCs was up-regulated, while it was down-regulated in chemerin knockdown VSMCs compared with the normal VSMCs .CONCLUSION: Chemerin pro-motes the proliferation of mouse vascular smooth muscle cells by up-regulating p-JNK production .
