Observation of estrous cycle in sexual maturation and ovariectomized fe-male ICR mice
10.3969/j.issn.1000-4718.2015.12.033
- VernacularTitle:去卵巢和性成熟期 ICR 雌性小鼠动情周期的观察
- Author:
Nana DU
;
Dandan XU
;
Lili HUANG
- Publication Type:Journal Article
- Keywords:
ICR mice;
Ovariectomy;
Estrous cycle;
Vaginal smear
- From:
Chinese Journal of Pathophysiology
2015;(12):2301-2304
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the characteristics of estrous cycle of female ICR mice and ovariectomized mice that had reached sexual maturity stage .METHODS:ICR mice were randomly divided into 3 groups, normal group, sham operation group and ovariectomized group .After ovariectomy and sham operating , at the 8th day, the mice were taken vagi-nal smears twice daily (09:00 and 16:00) for 12 consecutive days.The feature of vulva in the mice and vaginal smear cy-tology, occurrence rate of regular estrous cycle , duration of estrous cycle and each stage duration of estrous cycle were re -corded and observed .RESULTS:The characteristics of estrous cycle in normal group and sham operation group were simi-lar.Nearly 85%of the ICR mice in sexual maturity stage showed regular estrous cycle .The majority manifested 6-day cy-cle (83%), and only a small number exhibited 5-day cycle (17%).Compared with 5-day group, the duration of oestrus was significantly increased in 6-day group (P<0.05).The number and kind of the cells in vaginal smear were a little dif-ferent between 5-day and 6-day groups.The ovariectomized mice which showed irregular estrous cycle (76%) were charac-terized by continuous diestrus .CONCLUSION:Female ICR mice have the strain characteristics of estrus cycle .
