Effects of Bone Marrow Mesenchymal Stem Cells Transplantation on Expression of Growth Associated Protein-43 in Rats after Spinal Cord Injury
10.3969/j.issn.1006-9771.2015.12.006
- VernacularTitle:骨髓间充质干细胞静脉移植对脊髓损伤大鼠生长相关蛋白-43表达的影响
- Author:
Bingkui LI
;
Bin ZENG
;
Wei CHANG
;
Dayi WANG
;
Qi YANG
- Publication Type:Journal Article
- Keywords:
spinal cord injury;
bone marrow mesenchymal stem cells;
transplantation;
growth associated protein-43;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2015;(12):1391-1396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of growth associated protein-43 (GAP-43) in rats after spinal cord injury (SCI). Methods The SCI models were made by impacting at the level of T11 segment. After modeling, the rats were randomly and equally divided into control group (n=18) and observation group (n=18). 1 week after injury, DMEM/F12 medium 0.1 ml was injected into tail vein of rats in the control group, and an equal volume of BMSCs suspension was injected in the observation group. 1, 2, 4, 8 weeks after SCI, the hindlimb function was evaluated by the Basso-Beattie-Bresnahan (BBB) score. 2, 4, 8 weeks after SCI, the expression of GAP-43 mRNA was detected by RT-PCR, and the expression of GAP-43 was detect-ed by the immunohistochemistry. Results 2, 4, 8 weeks after SCI, compared with the control group, The BBB score increased, the expres-sion of GAP-43 mRNA and GAP-43 increased in the observation group (P<0.05). Conclusion BMSCs transplantation can improve the ex-pression of GAP-43 in gene and protein level, and then promote the repair of SCI.
