Protective effect of Salvianolic acid A against isoproterenol-induced myocardial infarction in mice
10.3969/j.issn.1001-1978.2015.12.009
- VernacularTitle:丹酚酸A对异丙肾上腺素致小鼠心肌缺血的保护作用及其机制
- Author:
Ziran NIU
;
Xiaona XU
;
Yucai CHEN
;
Huifang ZHANG
;
Yihuang LIN
;
Lianhua FANG
;
Guanhua DU
- Publication Type:Journal Article
- Keywords:
Salvianolic acid A;
isoproterenol;
myo-cardial infarction;
cell apoptosis;
anti-oxidation;
in-flammation;
PI3K/Akt signaling pathway
- From:
Chinese Pharmacological Bulletin
2015;(12):1667-1674
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of Salvianol-ic acid A (Sal A)on mice with isoproterenol (ISO)-induced myocardial infraction and its possible mecha-nisms.Methods The mice were subcutaneously in-jected with ISO (8 mg·kg-1 )to induce myocardial in-farction.The myocardial protective effect of Salvianolic acid A was evaluated from mortality rate,electrocardio-gram (ECG),heart function,myocardial infarction in-dex,serum myocardial enzymes and its action mecha-nisms were explored from inflammation,anti-oxidation and cells apoptosis.Results Salvianolic acid A dose-dependently enhanced the heart function of myocardial infarction mice,reduced the heart index,inhibited the myocardial enzyme leakage,showed obvious myocardi-al protection effects.ELISA results showed that Salvi-
anolic acid A could reduce the expression of myocardial inflammatory cytokines such as IL-6(interleukin-6,IL-6),TNF-α(tumornecrosis factor-α,TNF-α).West-ern-blotting confirmed that Salvianolic acid A could in-crease the expression of anti-apoptotic proteins Bcl-2, reduce the expression of apoptosis protein Bax,and raise the phosphorylation level of PI3K and Akt.Con-clusion Salvianolic acid A displays a significant pro-tective effect against isoproterenol-induced myocardial infarction and its mechanism may be related to the in-crease of PI3K/Akt signal pathway and the inhibition of cell apoptosis and inflammatory reaction.
