Establishment of an inactivated viral particle-based ELISA for the detection of anti-MERS-CoV anti-bodies
10.3760/cma.j.issn.0254-5101.2016.02.003
- VernacularTitle:基于全病毒颗粒的中东呼吸综合征冠状病毒抗体检测方法的建立
- Author:
Huijuan WANG
;
Wenling WANG
;
Jiaming LAN
;
Tie SONG
;
Wei ZHEN
;
Weimin ZHOU
;
Changwen KE
;
Guizhen WU
;
Wenjie TAN
- Publication Type:Journal Article
- Keywords:
Middle East respiratory syndrome;
Coronavirus;
Antibody;
ELISA
- From:
Chinese Journal of Microbiology and Immunology
2016;36(2):93-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an inactivated viral particle-based ELISA for the detection of antibodies against Middle East respiratory syndrome coronavirus (MERS-CoV) in serum samples collected from a MERS-CoV associated case. Methods Serum samples were collected from 10 newborns and 40 healthy adults. A viral particle-based ELISA was established by using the inactivated MERS-CoV virions as antigen. The levels of IgM and IgG antibodies in the serum samples were detected by the established ELISA and the cut-off values for positive detection were determined. Then the inactivated MERS-CoV virion-based ELISA was used to detect the antibodies against MERS-CoV in 5 serum samples collected from the first im-ported MERS case in China. Results The cut-off values of IgM and IgG antibodies in serum samples for ELISA were determined to be A450 readings of 0. 32 and 0. 42, respectively. The titers of IgM and IgG anti-bodies in serum samples collected at early admission to hospital from the first imported MERS case in China were both 1 ︰ 40. Seroconversion occurred 2 weeks after his admission to hospital with the titers of IgM and IgG reaching to 1 ︰ 320. Conclusion The inactivated MERS-CoV virion-based ELISA was established successfully and could be used for the detection of serum antibodies (IgG and IgM) in MERS associated cases.
