The establishment of chronic salpingitis model of New Zealand rabbits caused by Escherichia coli
10.3969.j.issn.1671-7856.2016.03.005
- VernacularTitle:大肠埃希菌致兔慢性输卵管炎症模型的建立
- Author:
Zicong FENG
;
Zhao ZHANG
;
Zhe LI
;
Boyi FENG
;
Siai CHEN
;
Junyu LI
;
Xiaomin XIAO
- Publication Type:Journal Article
- Keywords:
Chronic salpingitis model;
Mixed bacteria liquid;
E.coli liquid;
Trans-vaginal intrauterine intubation operation
- From:
Chinese Journal of Comparative Medicine
2016;26(3):19-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility of utilizing the mixed bacteria liquid and the Escherichia coli ( E.coli) liquid to establish the chronic salpingitis model of New Zealand rabbits, respectively.Methods Taken as the study object, the un-pregnant New Zealand rabbits (4~5 years old) were randomly divided into three groups: the normal group, the mixed bacteria experimental group and the E.coli experimental group.The trans-vaginal intrauterine intubation operation was performed for the injection of the mixed bacteria liquid and the E.coli liquid.Visual observation was to evaluate the gross pathological changes of the salpingitis and the pelvic cavity.HE staining and the light microscope were used to observe the micro-pathological changes of salpingitis.Results On the 15th day after modeling, increased pelvic effusion, dense peritoneal adhesion, interstitial hyperplasia and infiltration of lymphocytes were observed in both experimental groups. Conclusion Through the trans-vaginal intrauterine intubation operation, the chronic salpingitis model of New Zealand rabbits could be successfully established either by using the mixed bacteria liquid or by using the E.coli liquid.
