Effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of pulmonary adenocarcinoma A549 cells
10.11958/51942
- VernacularTitle:重组人白细胞介素-11对肺腺癌A549细胞增殖、迁移和侵袭的影响
- Author:
Rong XIAO
;
Mafei KANG
;
Meiqing LUO
;
Cuimei DONG
;
Xiuli LIU
- Publication Type:Journal Article
- Keywords:
interleukin-11;
carcinoma;
non-small-cell lung;
cell migration assays;
neoplasm invasiveness;
matrix me-talloproteinase 2;
matrix metalloproteinase 9;
rhIL-11
- From:
Tianjin Medical Journal
2016;44(1):46-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of recombinant human interleukin 11(rhIL-11) on proliferation, migration and invasion of A549 cells, and the mechanism thereof. Methods Final concentrations of 0, 10, 20, 50 and 100μg/L rhIL-11 were added into pulmonary adenocarcinoma A549 cells. The cell proliferation was detected by MTT. The wound-healing, transwell migration assay were used to validate the capability of the migration and invasion of A549 cells. Matrix metallopro-teinases (MMP)-2 and MMP-9 protein expressions were revealed by Western blot assay. Results The proliferation of A549 cells was not significantly changed by rhIL-11. The cell capability to migrate and invade was significantly increased 24 h af-ter treatment with rhIL-11 (P<0.05). The expression levels of MMP-2 and MMP-9 were significantly un-regulated, and which were increased with the increased concentrations of rhIL-11 (P<0.05). Conclusion rhIL-11 can promote the migra-tion and invasion of A549 cells, and the up-regulation of MMP-2 and MMP-9 expression might be one of the mechanisms.
