Influence of miR-21 control Smad7 expression and lung cancer A549 cell line proliferation
- VernacularTitle:microRNA-21对肺癌A549细胞增殖和Smad7表达的影响
- Author:
Bin LI
;
Yueyin PAN
;
Yingying DU
- Publication Type:Journal Article
- Keywords:
lung cancer;
microRNA-21;
Smad7;
proliferation
- From:
Acta Universitatis Medicinalis Anhui
2015;50(12):1766-1769
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore miR-21 regulation of Smad7 in lung cancer A549 cell line as well as its impact on the proliferation of lung cancer A549 cell line. Methods miR-21 mimic and inhibitors in lung cancer A549 cell line were transfected by using Lipofectamine 2000 Reagent. After 48 h, Western blot and qRT-PCR were applied to assess the expression of protein and mRNA of Smad7 . MTT assay was used to determine the proliferation influence of the transfected lung cancer A549 cell line. Results Western blot and qRT-PCR showed that A549 cell trans-fected miR-21 mimic exhibited down-regulated Smad7 protein and mRNA expression, and A549 cell transfected miR-21 inhibitor exhibited up-regulated Smad7 protein and mRNA expression. The A549 cell proliferation activity decreased significantly after transfected miR-21 inhibitors. Conclusion miR-21 inhibitors can increase Smad7 pro-tein and mRNA expression, and suppress the proliferation activity of lung cancer A549 cell significantly.
