Effects of limb ischemic postconditioning on blood brain barrier of cerebral ischemia-reperfusion injury of mice and its mechanism
- VernacularTitle:肢体缺血后适应对小鼠脑缺血再灌注损伤后血-脑屏障的影响及机制
- Author:
Pan LI
;
Xiaofang LI
;
Likai SU
- Publication Type:Journal Article
- Keywords:
cerebral ischemia;
limb ischemic postconditioning;
blood brain barrier;
claudin-5;
occludin
- From:
Journal of Clinical Neurology
2015;28(6):445-448
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Limb ischemic postconditioning ( LIPostC) on blood brain barrier (BBB) and explore the influence on the the expression of tight junction protein claudin-5,occludin in cerebral ischemia-reperfusion injury of mice.Methods Ninety male CD1 mice were divided into three groups randomly:Sham-operated group(sham group), ischemia-reperfusion group(I/R group) and LIPostC group.Mouse middle cerebral artery cerebral ischemia-reperfusion model was established by modified-occlusion method. Neurological deficit scores, brain water content, infarct volume, BBB dysfunction were measured at 24 h after reperfusion.Western blot and RT-qPCR were used to analyze the expressions of claudin-5, occludin.Results Compared with I/R group, neurological deficits scores were significantly decreased, brain edema was relieved, infarct volume was reduced, and the expression of claudin-5, occludin up-regulated, the differences were statistically significant(all P<0.05).Conclusion LIPostC can protect the BBB of cerebral ischemia-reperfusion injury mice by increasing the expression of claudin-5,occludin, and that will provide a theoretical basis for its clinical application.
