Effects of Different Extracts ofTangwang Mingmu Granules on High Glucose Induced VEGF and IL-1α Expressions in Vascular Endothelial Cells
10.3969/j.issn.1005-5304.2016.01.013
- VernacularTitle:糖网明目颗粒不同提取部位对高糖诱导的血管内皮细胞中血管内皮细胞生长因子和白细胞介素-1α的影响
- Author:
Changying HAO
;
Mingxia CHEN
;
Wenbin MA
;
Ping GUO
;
Haibo LV
;
Ye LIU
;
Zenglin LIAN
- Publication Type:Journal Article
- Keywords:
Tangwang MingmuGranules;
diabetic retinopathy;
human umbilical vein endothelial cells EA.hy926;
VEGF;
IL-1α
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the mechanism of action of different extracts ofTangwang Mingmu Granules on high glucose induced VEGF and IL-1α gene and protein expressions in vascular endothelial cells.Methods Human umbilical vein endothelial cells EA.hy926 were divided into six groups: blank, high glucose,Tangwang Mingmu Granules, extract 1 (glycoside and flavonoid), extract 2 (organic acid and polysaccharides) and extract 3 (alkaloids) groups. High concentration glucose was used to establish the high glucose model in EA.hy926 cells. The expressions of VEGF and IL-1α mRNA were detected by semi-quantitative RT-PCR. The contents of VEGF and IL-1α protein were tested by ELISA.Results The gene expression and protein levels of VEGF and IL-1α were significantly up-regulated under the high glucose condition (P<0.05). However, the above indicators were significantly reduced after the treatment ofTangwang Mingmu Granules. The activity of different parts ofTangwang Mingmu Granules was as follows: extract 1> extract 3> extract 2.Conclusion The action intensity of glycosides and flavonoids, alkaloids, organic acids and polysaccharides on VEGF and IL-1α expression inTangwang Mingmu Granules weakens in sequence.
