Effects of Intra-articular Injection of Sinomenine on Morphology, MMP-13 Level and Cartilage Oligomeric Matrix Protein of Rabbit Knee Osteoarthritis Model
10.3969/j.issn.1005-5304.2016.01.017
- VernacularTitle:青藤碱关节腔注射对膝骨关节炎兔软骨和滑膜组织形态学及基质金属蛋白酶-13和软骨寡聚基质蛋白的影响
- Author:
Jie ZHENG
;
Ruihui WANG
;
Jiushe KOU
- Publication Type:Journal Article
- Keywords:
sinomenine;
osteoarthritis;
morphology;
MMP-13;
cartilage oligomeric matrix protein;
rabbits
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;(1):74-77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of intra-articular injection of sinomenine on morphology of synovium and cartilage as well as contents of MMP-13 and cartilage oligomeric matrix protein(COMP) in serum and synovial fluid of rabbit knee osteoarthritis model.Methods A total of 38 New Zealand white rabbits were randomly divided into 4 groups: control group, model group, hyaluronate group and sinomenine group. Model group, hyaluronate group and sinomenine group established knee osteoarthritis model by intra-articular injection of papain enzyme, and were treated with saline, sodium hyaluronate and sinomenine respectively except for control group. 5 weeks after treatment, all rabbits were sacrificed for HE staining and histological grading on cartilago articularis and synovium, and ELISA method was used to detect the contents of MMP-13 and COMP in rabbit serum and synovial fluid.Results Mankin's scores of articular cartilage and histological scores of synovium in model group were significantly higher than those of control group (P<0.01), and were markedly lower in sinomenine group than those of model group (P<0.01); the contents of MMP-13 and COMP in serum and synovial fluid of model group were significantly raised compared with control group (P<0.01), and were reduced obviously in sinomenine group compared with model group (P<0.05,P<0.01).Conclusion Intra-articular injection of sinomenine can reduce the levels of MMP-13 and COMP in serum and synovial fluid of rabbit knee osteoarthritis model, and improve synovial inflammation, as well as delay the degradation of articular cartilage.
