Study on HPLC Fingerprint ofWanbiqing Pills
10.3969/j.issn.1005-5304.2016.01.023
- VernacularTitle:顽痹清丸HPLC指纹图谱研究
- Author:
Shiren LUO
;
Zhao WANG
;
Jie LI
- Publication Type:Journal Article
- Keywords:
Wanbiqing Pills;
HPLC;
fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;(1):96-99
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an evaluation system of HPLC fingerprint of Wanbiqing Pills; To provide references for the quality control ofWanbiqing Pills.Methods Wanbiqing Pills were analyzed with HPLC method. Analysis was performed on Agilent C18 column (4.6 mm×250 mm, 5μm) with the mobile phase of acetonitrile- 0.1%H3PO4 water gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 258 nm. Sample injection was 20μL. Standard fingerprint ofWanbiqing Pills was established based on Similarity Evaluation System for Chromatographic Fingerprint of TCM (Version 2004 A). The similarity ofWanbiqing Pills from different batches was computed.Results A standard HPLC fingerprint procedure was developed forWanbiqing Pills with 20 common peaks. The similarity of 10 batches ofWanbiqing Pills was not lower than 0.98. Five peaks received chemical confirmation.Conclusion The HPLC method is stable and feasible and with good repeatability, which can be used for the quality control ofWanbiqing Pills.
