Study on Quality Standards forAnshu Liquid
10.3969/j.issn.1005-5304.2016.01.024
- VernacularTitle:安舒液质量标准研究
- Author:
Dongshun LIU
;
Wenquan LU
;
Shujuan PIAO
;
Xiaojuan XIONG
- Publication Type:Journal Article
- Keywords:
Anshu Liquid;
berberine hydrochloride;
phellodendrine hydrochloride;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;(1):100-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish quality standards forAnshu Liquid.Methods Phellodendri Chinensis Cortex and Kochiae Fructus were qualitatively identified by TLC method. The contents of berberine hydrochloride and phellodendrine hydrochloride in the preparation were determined by HPLC method. The chromatographic column was ZORBAX SB-C18 (4.6 mm×250 mm, 5μm); the mobile phase was acetonitrile-0.1% phosphoric acid (0.5% diethylamine) gradient elution; the flow rate was 1.0 mL/min; the detection wavelength was 284 nm.Results Berberine hydrochloride and phellodendrine hydrochloride in Phellodendri Chinensis Cortex and saponin Ic in Kochiae Fructus were detected by TLC method. The linear relationship of berberine hydrochloride in the preparation was Y=23.109X?30.548,r2=0.999 8, RSD=2.97%, showing that the linear range of berberine hydrochloride was 0.050 5– 2.525 0 μg. The linear relationship of phellodendrine hydrochloride in the preparation wasY=10.038X?2.446 6, r2=0.999 9, RSD=1.24%, showing that the linear range of phellodendrine hydrochloride was 0.050 0–2.500 0 μg. Conclusion The method is accurate, rapid, stable and reliable, with good reproducibility, which can be used for the quality control and evaluation ofAnshu Liquid.
