Effects ofXiangsha LiujunziDecoction on Gastric Emptying Function, Pepsin Activity and Expression of HIF-1α in Chronic Atrophic Gastritis of Rats with Deficiency Spleen and Stomach
10.3969/j.issn.1005-5304.2016.01.011
- VernacularTitle:香砂六君子汤对脾胃虚弱型萎缩性胃炎大鼠胃排空功能及胃蛋白酶活性和缺氧诱导因子-1α表达的影响
- Author:
Yunyan DUAN
;
Yingxia CHENG
;
Qiang WANG
;
Lanzhen LI
;
Qingsheng WANG
;
Shiduo ZHENG
;
Pengcheng LU
;
Zuohan LEI
;
Xuesong LIU
;
Zhenhua LIU
- Publication Type:Journal Article
- Keywords:
gastric emptying function;
pepsin;
HIF-1α;
atrophic gastritis;
Xiangsha LiujunziDecoction;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2016;(1):47-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study effects ofXiangsha Liujunzi Decoction (XSLJZ) on gastric emptying function, pepsin activity and expression of HIF-1α gene and protein of gastric mucosa in chronic atrophic gastritis (CAG) of rats with deficiency of spleen and stomach.Methods Rats were randomly divided into normal group and model group. The models of CAG rats with deficiency of spleen and stomach type were induced by synthetic methods. After the modeling, models rats were divided into model group, positive control group and XSLJZ high-, medium-, and low-dose groups. Normal group and model group were given 10 mL/kg distilled water every day for gavage; XSLJZ high-, medium-, and low-dose groups were given 24, 12, and 6 g/kg XSLJZ Decoction for gavage; positive control group was given 0.30 g/kg Vatacoenayme for gavage, for successive 120 d. Gastric emptying function and pepsin activity were detected, and HIF-1α gene and protein expression in gastric mucosa were detected by RT-qPCR and IHC. Results Compared with the normal group, the gastric emptying function and pepsin activity in the model group were much lower (P<0.01); expressions of HIF-1α gene and protein in gastric tissues was much higher (P<0.01). Compared with model group, XSLJZ could increase the gastric emptying function and pepsin activity significantly (P<0.05), and decrease expressions of HIF-1α gene and protein (P<0.05).Conclusion XSLJZ has functions of improving the gastric emptying function, promoting pepsin activity, and reducing expressions of HIF-1α gene and protein.
