Bone marrow mesenchymal stem cell transplantation for liver cirrhosis in rats：variation of liver ultrastructure, stereology parameters and liver function indexes
10.3969/j.issn.2095-4344.2015.50.019
- VernacularTitle:骨髓间充质干细胞移植肝硬化大鼠肝脏超微结构、体视学参数和肝功能的变化
- Author:
Tilong DING
;
Yanchun MA
;
Li YU
;
Yong MA
- Publication Type:Journal Article
- Keywords:
Liver Cirrhosis;
Bone Marrow;
Mesenchymal Stem Cel Transplantation;
Tissue Engineering
- From:
Chinese Journal of Tissue Engineering Research
2015;(50):8144-8148
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cel transplantation has a certain controversy in the treatment of liver cirrhosis, and its effects on the receptor liver structure and function need further studies.
OBJECTIVE:To study the changes in liver ultrastructure, stereology parameters and liver function indexes of rat models with liver cirrhosis treated by bone marrow mesenchymal stem cel transplantation.
METHODS:Rat models of liver cirrhosis were made using carbon tetrachloride and treated by bone marrow mesenchymal stem cel transplantation. Liver ultrastructure of al the rats were observed by transmission electron microscope, the stereology parameters of the hepatic sinusoid were analyzed by a stereology analysis software, and the serum liver function indexes were detected by a biochemical analyzer.
RESULTS AND CONCLUSION: (1) The hepatic cels in the rat models exhibited acute hypoxia, lots of mitochondria were destroyed, and obvious karyopycnosis and capilarization of the hepatic sinusoid were found. The liver ultrastructure of rats undergoing cel transplantation was improved remarkably, the hepatic cel nucleus was nearly normal, mitochondrial sweling relieved notably and nuclear pore clogging lessened. (2) The number of hepatic sinusoids in the model group was reduced dramaticaly, but the total area and mean diameter of the hepatic sinusoid were enlarged significantly as compared with the cel transplantation and normal groups (P < 0.05). (3) Compared with the model group, the albumin level was significantly increased, but the levels of alanine aminotransferase and direct bilirubin were significantly decreased in the cel transplantation (P < 0.05). In addition, there was no significant difference in the level of aspartate aminotransferase between the model and cel transplantation groups (P > 0.05). These findings indicate that bone marrow mesenchymal stem cel transplantation can improve liver function and structure of rats with liver cirrhosis and lessen pathological changes of hepatic sinusoid, so it is an effective treatment for liver cirrhosis.
