Mechanism of genuineness of Glycyrrhiza uralensis based on SNP of β-Amyrin synthase gene.
- Author:
Yimei ZANG
;
Yanpeng LI
;
Jing QIAO
;
Honghao CHEN
;
Chunsheng LIU
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2015;50(7):906-9
- CountryChina
- Language:Chinese
-
Abstract:
β-Amyrin synthase (β-AS) genes of Glycyrrhiza uralensis from 6 different regions were analyzed by PCR-SSCP and sequenced, then the correlationship between β-AS SNP and regions of Glycyrrhiza uralensis were determined. According to the 1 coding single nucleotide polymorphism on the first exon of β-AS gene at 94 bp site, Glycyrrhiza uralensis could be divided into 3 genotypes. In these genotypes, the percentage of 94A type in genuine regions was much higher, and it had significant differences with the percentage in non-genuine regions (P < 0.001). The results of the experiment proved that different β-AS genotypes at 94 bp site from different regions may be one of the important reasons to result in the genuineness of Glycyrrhiza uralensis.