In vitro Stability of Heat Shock Protein 27 in Serum and Plasma Under Different Pre-analytical Conditions: Implications for Large-Scale Clinical Studies.
10.3343/alm.2016.36.4.353
- Author:
Matthias ZIMMERMANN
1
;
Denise TRAXLER
;
Elisabeth SIMADER
;
Christine BEKOS
;
Benjamin DIEPLINGER
;
Mitja LAINSCAK
;
Hendrik Jan ANKERSMIT
;
Thomas MUELLER
Author Information
1. Christian Doppler Laboratory for Cardiac and Thoracic Diagnosis and Regeneration, Medical University of Vienna, Vienna, Austria.
- Publication Type:Brief Communication
- Keywords:
Heat shock proteins;
In vitro stability;
Storage conditions
- MeSH:
*Enzyme-Linked Immunosorbent Assay;
Freezing;
HSP27 Heat-Shock Proteins/*blood;
Humans;
Protein Stability;
Reproducibility of Results;
Specimen Handling;
Temperature;
Time Factors
- From:Annals of Laboratory Medicine
2016;36(4):353-357
- CountryRepublic of Korea
- Language:English
-
Abstract:
The effects of storage temperatures, repeated freeze-thaw cycles, or delays in separating plasma or serum from blood samples are largely unknown for heat shock protein 27 (HSP27). We evaluated (1) the imprecision of the HSP27 assay used in this study; (2) the in vitro stability of HSP27 in blood samples stored at 4℃ for up to 6 hr with immediate and delayed serum/plasma separation from cells; and (3) the in vitro stability of HSP27 in blood samples stored at -80℃ after repeated freeze-thaw cycles. The ELISA to detect HSP27 in this study showed a within-run CV of <9% and a total CV of <15%. After 4-6 hr of storage at 4℃, HSP27 concentrations remained stable when using serum tubes irrespective of sample handling, but HSP27 concentrations decreased by 25-45% when using EDTA plasma tubes. Compared with baseline HSP27, one freeze-thaw cycle had no effect on serum concentrations. However, plasma concentrations increased by 3.1-fold after one freeze-thaw cycle and by 7.3-fold after five freeze-thaw cycles. In conclusion, serum is an appropriate biological sample type for use in epidemiological and large-scale clinical studies.
