Effects of different durations of severe acute hypervolemic hemodilution on acute lung injury in rabbits
10.3760/cma.j.issn.0254-1416.2015.09.025
- VernacularTitle:不同时程深度急性高容血液稀释对兔急性肺损伤的影响
- Author:
Lihong ZHANG
;
Xiaoni ZHANG
;
Weiqiang ZHUANG
- Publication Type:Journal Article
- Keywords:
Hemodilution;
Respiratory distress syndrome,adult
- From:
Chinese Journal of Anesthesiology
2015;35(9):1121-1123
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of different durations of severe acute hypervolemic hemodilution (AHH) on acute lung injury in rabbits.Methods Thirty-six healthy adult rabbits of both sexes, weighing 2.7-3.1 kg, were randomly divided into 2 groups (n=18 each) using a random number table: control group (C group) and AHH group.AHH was performed with 6％ hydroxyethyl starch 130/0.4 until the target hematocrit of 20％ was achieved in group AHH.Immediately before AHH (T0) , and at 1,2 and 4 h after AHH (T1-3) , blood samples were taken from the right femoral artery for blood gas analysis.The oxygenation index was calculated.The occurrence of acute lung injury (oxygenation index ≤ 300 mm-Hg) was recorded.After blood samples were collected at T1-3 , 6 rabbits in each group were sacrificed, and lungs were removed for determination of wet/dry lung weight ratio (W/D ratio) and aquaporin-1 (AQP-1) expression (by immuno-histochemistry).Results In group AHH, 1 rabbit developed acute lung injury at 1-2 h after AHH, and 4 rabbits developed acute lung injury at 2-4 h after AHH.Compared with group C,W/D ratio was significantly increased at T1-3, and the expression of AQP-1 was down-regulated at T2,3 in group AHH (P＜0.05 or 0.01).Conclusion Severe AHH performed with 6％ hydroxyethyl starch 130/ 0.4 with the target hematocrit reduced to 20％ and the duration ＞ 1 h induces acute lung injury in rabbits.
