Detection techniques for calreticulin gene mutation and the clinical application
10.3760/cma.j.issn.1009-9158.2015.11.017
- VernacularTitle:钙网蛋白基因突变检测技术及临床应用选择
- Author:
Yuqing PEI
;
Fei WANG
;
Wei CUI
- Publication Type:Journal Article
- Keywords:
Myeloproliferative disorders;
Calreticulin;
Mutation;
Polymerase chain reaction;
High resolution melting;
Sequence analysis,DNA
- From:
Chinese Journal of Laboratory Medicine
2015;38(11):782-785
- CountryChina
- Language:Chinese
-
Abstract:
The mutation status of calreticulin gene (CALR) is helpful for the diagnosis of JAK2 / MPL mutation-negative myeloproliferative neoplasms (MPN), and is closely related to the MPN progression and prognosis.Currently, Sanger sequencing, PCR-fragment analysis, high resolution melting, Taqman RQPCRand the next generation sequencing have been reported to be used to detecting the CALR gene mutations.A proper method for CALR mutation detection and a right laboratory diagnostic procedure according to the MPN-related molecular markers will facilitate the rapid and effective diagnosis and treatment of MPN.
