Role of heparanase in endotoxemia-induced destruction of vascular endothelial glycocalyx in rats
10.3760/cma.j.issn.0254-1416.2015.09.028
- VernacularTitle:肝素酶在内毒素血症致大鼠血管内皮糖萼破坏中的作用
- Author:
Tingting JIANG
;
Jiafeng SUN
;
Jiangyan XIA
;
Xiaodong QIU
;
Jing YUAN
;
Xinjian LU
;
Yongying SUN
- Publication Type:Journal Article
- Keywords:
Heparin lyase;
Endotoxemia;
Glycocalyx;
Endothelial cells
- From:
Chinese Journal of Anesthesiology
2015;35(9):1131-1133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the role of heparanase in endotoxemia-induced destruction of vascular endothelial glycocalyx in rats.Methods Forty-eight pathogen-free male Sprague-Dawley rats, aged 8-10 weeks, weighing 200-250 g, were randomly divided into 3 groups (n=16 each) using a random number table: control group (group C), lipopolysaccharide (LPS) group (group L) and LPS+ heparin group (group LH).Endotoxemia was induced by intravenous LPS 15 mg/kg in L and LH groups, and heparin sodium 100 U · kg-1 · h-1 was infused simultaneously in group LH.At 3 and 6 h after LPS injection, blood samples were collected from the femoral vein for determination of the serum concentrations of serum heparin sulfate (HS), syndecan-1, E-selectin and intercellular adhesion molecule-1 (ICAM-1) concentrations.The rats were then sacrificed, and lungs were removed for microscopic examination and for determination of wet/dry lung weight ratio (W/D ratio).Results Compared with group C, the serum HS, syndecan-1, E-selectin and ICAM-1 concentrations and W/D ratio were significantly increased in group L, and the serum HS and ICAM-1 concentrations were increased in group LH (P＜0.05).Compared with group L, the serum HS, syndecan-1, E-selectin and ICAM-1 concentrations and W/D ratio were significantly decreased in group LH (P＜0.05).The pathological changes of lungs were obvious in group L, and were significantly mitigated in group LH.Conclusion Heparanase is involved in endotoxemiainduced destruction of vascular endothelial glycocalyx in rats.
