Study on the correlation between ultrasound imaging and pathology in acute lung injury of rats
10.3760/cma.j.issn.1004-4477.2015.10.021
- VernacularTitle:大鼠急性肺损伤模型超声成像与病理的相关性研究
- Author:
Weiying ZHAO
;
Guoyuan XIA
;
Liang DONG
;
Jiangang SUN
;
Qingmeng LIU
;
Xueyan SHEN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Acute lung injury
- From:
Chinese Journal of Ultrasonography
2015;24(10):898-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the correlation between ultrasonography and histopathology in acute lung injury of rats.Methods Twenty male Wistar rats were randomly divided into control group (4 rats) and experimental group (16 rats),in the experimental group acute lung injury models were step-by-step induced by oleic acid (OA) and sequentially by OA and lipopolysaccharide (LPS).After the end of each step of models,ultrasound examination was applied,and the rats were sacrificed to take the lung specimens to be observed.The ultrasonography and pathological results were analyzed,and the numbers of alveolar were counted under microscope (200 times).Results With the aggravation of acute lung injury,the sonographic findings showed unclear or disappeared pleural line,close-set of B-lines,and pulmonary consolidation with air bronchogram.For control group,the ultrasonographic score was 0.25 ± 0.50,lung injury pathological score was 0.50 ± 0.58,and the numbers of alveolar were 25 ± 3.For OA group,the ultrasonographic score was 2.86-± 1.35,lung injury pathological score was 6.28-± 0.76,and the numbers of alveolar were 10-± 2.For OA + LPS group,the ultrasonographic score was 5.83-± 2.32,lung injury pathological score was 9.83 ± 0.98,and the numbers of alveolar were 6-± 2.All 3 groups were significant different on ultrasonographic score,pathological score and alveolar counts (P ＜ 0.01).Conclusions The ultrasonography image of acute lung injury of rats were positively correlated with its histopathological alteration.The ultrasonography can effectively evaluate the degree of acute lung injury of rats.
