β-adrenoceptor activation induces the apoptosis of human mesangial cells
10.3760/cma.j.issn.1001-7097.2015.07.007
- VernacularTitle:β肾上腺素受体激活诱导人系膜细胞凋亡
- Author:
Fanwu KONG
;
Yubao LIU
;
Lijie LIANG
;
Qiushuang LIU
;
Yeping REN
- Publication Type:Journal Article
- Keywords:
Mesangial cell;
Sympathetic nerve system;
Apoptosis;
Oxidative stress
- From:
Chinese Journal of Nephrology
2015;31(7):516-520
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of β-adrenoceptor (β-AR) activation on the apoptosis in human mesangial cells and it's mechanism.Methods Cultured HMC were used in experiments and were divied into four groups:the control group; β-AR activation (β-AR agonist NE/Pra) group; β-AR inhibitor (Prop) group; antioxidants group.The experiments technology including PCR,confocal scanning microscope,immunofluorescence and Tunel.Results The results of RTPCR and confocal scanning microscope showed that β1-AR and β2-AR were expressed in human HMC.β-AR activation induced reactive oxygen species (ROS) increase in human MCs,the relative levels of ROS were elevated as early as 0.5 h after β-AR activation,and gradually increased and peaked at 4 h on a concentration and time dependent manner.Tunel results demonstrated that β-AR activation induced apoptosis with ROS on a concentration and time dependent manner,β-AR blocking agent-propranolol significantly inhibited β-AR activation induced apoptosis.Antioxidants including vitamin C and NAC could inhibited β-AR activation induced apoptosis (all P ＜ 0.01).Conclusions β-AR is functionally expressed in human mesangial cell,furthermore β-AR activation-induced ROS increase mediate apoptosis.Antioxidants can inhibit β-AR activation induced apoptosis.
