Analysis of miRNA-34c expression in cervical cancer tissue and preliminary identification of Polo-like kinase 4 as its target gene
10.3969/j.issn.1000-8179.2015.22.028
- VernacularTitle:宫颈癌组织中miRNA-34c的表达水平分析及其靶基因PLK4的鉴定
- Author:
Honghong ZHANG
;
Xiaoli LIU
;
Sijuan XU
- Publication Type:Journal Article
- Keywords:
miRNA-34c;
PLK4;
cervical cancer
- From:
Chinese Journal of Clinical Oncology
2015;42(22):1077-1080
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the expression profile of miRNA-34c in cervical cancer tissue and identify its novel target gene. Methods:The expression levels of miRNA-34c were detected in 34 paired cervical cancer tissues and normal paraneoplastic tissues via quantitative reverse transcription polymerase chain reaction (qRT-PCR). Polo-like kinase 4 (PLK4) is a target gene of miRNA-34c pre-dicted in the miRNA Target Database. Luciferase vector containing the binding site of miRNA-34c to PLK4 3'UTR and miRNA-34c mimic or negative control were co-transfected into HEK293T cells, and luciferase expression was examined. The miRNA-34c mimic or negative control was transfected into SiHa cells, and the mRNA or protein expression of PLK4 was detected via qRT-PCR or Western blot, respectively. Results:MiRNA-34c expression was lower in cervical cancer tissues than in normal paraneoplastic tissues. The miR-NA-34c mimics significantly inhibited luciferase activation in the HEK293T cells (P<0.01) and significantly decreased the mRNA and protein expression levels of PLK4 in the SiHa cells (P<0.01). Conclusion:MiRNA-34c is significantly decreased in cervical cancer tis-sues. Moreover, miRNA-34c can significantly repress the mRNA and protein expression levels of PLK4 by directly targeting the 3'UTR.
