Study on the mechanism of homocysteine-induced microglia expression of interleukin-1β and tumor necrosis factor-α
10.3760/cma.j.issn.0254-9026.2015.08.020
- VernacularTitle:同型半胱氨酸诱导小鼠胶质细胞表达白介素-1β和肿瘤坏死因子-α的研究
- Author:
Ying LI
;
Ruiyang MAO
;
Xiaohong DU
;
Xiaowei ZHAO
- Publication Type:Journal Article
- Keywords:
Hyperhomocysteinemia;
Interleukin-beta;
Tumor necrosis factor-alpha;
NF-kappaB
- From:
Chinese Journal of Geriatrics
2015;34(8):898-900
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of homocysteine-induced microglia (BV-2 cells) expression of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α).Methods The BV-2 cells were divided into blank control group,cysteine (Cys) group,homocysteine (Hcy) group and homocysteine and glutathione (Hcy+GSH) group,and the BV-2 cells in these groups were incubated with cysteine or homocysteine or homocysteine and glutathione together for 72 h.The mRNA expressions of IL-1β and TNF-α were assessed by RT-qPCR.The protein expressions of IL-1β and TNF-α in supernatant were detected by enzyme linked immunosorbent assay (ELISA).Western blot was used to observe the changes of NF-κB/p65 expression.Results There were significant differences in mRNA and protein expressions of IL-1β and TNF-α and NF-κB/p65 protein expression between groups (F=48.63,130.76,702.91,293.69,212.06,respectively,all P=0.000).The secretions of IL-1β and TNF-α were significantly improved by homocysteine (P＜0.05),and were reversed by the treatment with glutathione (P＜0.05).Western blot assay result showed that NF-κB/p65 was elevated after treatment with homocysteine (P＜0.05).Conclusions Homocysteine can induce microglia expression of interleukin-1β and tumor necrosis factor-α,and NF-κB signaling pathway may be involved in this process.
