Automated synthesis and quality analysis of 18F-FMISO based on CFN-MPS-100 module
10.3760/cma.j.issn.2095-2848.2015.04.016
- VernacularTitle:基于CFN-MPS-100模块的18F-FMISO自动化合成与质量分析
- Author:
Liping CHEN
;
Yu ZHANG
;
Najing WU
;
Xuyang YOU
;
Liang CHENG
;
Weixing WAN
- Publication Type:Journal Article
- Keywords:
FMISO;
Isotope labeling;
Fluorine radiotopes;
Chemical synthesis
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2015;35(4):303-305
- CountryChina
- Language:Chinese
-
Abstract:
Objective To synthesize 18F-FMISO and analyze the quality of the product.Methods 1-(2'-nitro-l'-imidazolyl)-2-O-tetrahydropyranyl-O-trluendulfonylpropanediol (NITIT) was taken as the precursor and simple one pot method was used.CFN-MPS-100 fluorine multifunction radiopharmaceutical chemical synthesis module was adopted to complete the radioactive fluorination reaction in a closed flat flask,and the crude product was purified by semi-preparative HPLC,the solvent was removed by rotary evaporation.Then 15 ml saline was added into the product to get 18F-FMISO injection.Radio-HPLC and radio-TLC were applied for quality control.Results 18F-FMISO was obtained in 60 min with the radiochemical yield of (32±5.0)％ (no decay corrected,n=25).The radiochemical purity was above 99.0％ and still above 98.5％ after 6 h.The radioactive concentration was above 1.11 × 1012 Bq/L.The product was colorless solution,with pH value of 7.0.The radioactive nuclear purity was more than 99％.The K222 was less than 25 μg/ml.Conclusion 18 F-FMISO could be synthesized with automatic synthesis method based on the CFN-MPS-100 fluorine multifunction module.The labeling rate,stability and chemical purity are high.
