The study of new targeted-delivery of micro RNAs to bone-metastatic prostate tumors
10.3760/cma.j.issn.1008-1372.2015.08.016
- VernacularTitle:新型骨靶向纳米复合物抗前列腺癌骨转移细胞增殖作用研究
- Author:
Zhao HAO
;
Zhihong LI
- Publication Type:Journal Article
- Keywords:
MicroRNAs/PD;
Nanocomposites/TU;
Prostatic neoplasms/DT;
Neoplasm metastasis/DT;
Bone neoplasms/SC/DT
- From:
Journal of Chinese Physician
2015;17(8):1174-1178
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a new gene delivery system based on atelocollagen (ATE),and explore that modified aptamer (APT),and APT-ATE/miRNA (miRNA-15a and miRNA-16-1) were successfully synthesized to treat bone-metastatic prostatic cancers.Methods Flow cytometry (FCM) analysis was used to characterize APT-ATE complex.The diameter and zeta potential of complexes were measured by Zetasizer Nano-ZS9.The prostatic cancer (PCa) distribution experiments were used to explore its biological characteristics and targeting ability of PCa cells (PC3 and LNCaP).The inhibition of APT-ATE complex on LNCaP cell was determined with the cholecystokinin (CCK)-8 assay.Results FCM results demonstrated the successful synthesis of ATE-APT complex.The cellular uptake of vectors was concentration-dependent.The gene expression in vitro indicated that the modification of APT could increase the efficiency of gene expression and PCa targeting ability of ATE vectors to LNCaP [prostate specific membrane antigen (PSMA) over-expressing prostate cancer cells].The result of biodistribution showed that the bone uptake of APT-ATE was higher than ATE-APT.Conclusions APT-ATE/miRNA might be useful for preclinical and clinical studies on the treatment of bone-metastatic PCa.
