Study on the inhibitory effect of the black garlic extract on the growth of hela cells

VernacularTitle:黑蒜提取液对宫颈癌HeLa细胞株的生长抑制作用研究
Author: Ying LI ; Guiqing YANG ; Qun ZHOU ; Yishan WANG
Publication Type:Journal Article
Keywords: Garlic; Uterine cervical neoplasms/DT/ME; Proto-oncogene proteins c-bcl-2/ME; bcl-X protein/ME
From: Journal of Chinese Physician 2015;17(8):1167-1169
CountryChina
Language:Chinese
Abstract: Objective To explore the effect of black garlic extract on HeLa cells and its possible molecular mechanisms.Methods Inhibitory rate of HeLa cells was detected with methyl thiazolyl tetrazolium(MTT).Flow cytometry annexin V-fluorescein isothiocyanate/propidium iodide (V-FITC/PI) was used to detect tumor cell apoptosis.Flow cytometry was used to measure cell-cycle changes.Immunohistochemistry method was used to detect expressions of tumor proteins bax and Bcl-2.Results Black garlic extract significantly inhibited the growth of HeLa cells.Black garlic extract significantly induced apoptosis of HeLa cells.The apoptosis rate was (53.26 ± 1.78)％ in the black garlic high-dose group,and (3.68 ±0.11)％ in the control group.Black garlic extract affected cell cycle,upregulated bax expression,and downregulated bcl-2 expression.Conclusions Black garlic extract significantly induced the apoptosis of HeLa cells.This effect might be caused through increasing G2/M cells or changing expressions of bax and bcl-2.