Study on the inhibitory effect of the black garlic extract on the growth of hela cells
10.3760/cma.j.issn.1008-1372.2015.08.014
- VernacularTitle:黑蒜提取液对宫颈癌HeLa细胞株的生长抑制作用研究
- Author:
Ying LI
;
Guiqing YANG
;
Qun ZHOU
;
Yishan WANG
- Publication Type:Journal Article
- Keywords:
Garlic;
Uterine cervical neoplasms/DT/ME;
Proto-oncogene proteins c-bcl-2/ME;
bcl-X protein/ME
- From:
Journal of Chinese Physician
2015;17(8):1167-1169
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of black garlic extract on HeLa cells and its possible molecular mechanisms.Methods Inhibitory rate of HeLa cells was detected with methyl thiazolyl tetrazolium(MTT).Flow cytometry annexin V-fluorescein isothiocyanate/propidium iodide (V-FITC/PI) was used to detect tumor cell apoptosis.Flow cytometry was used to measure cell-cycle changes.Immunohistochemistry method was used to detect expressions of tumor proteins bax and Bcl-2.Results Black garlic extract significantly inhibited the growth of HeLa cells.Black garlic extract significantly induced apoptosis of HeLa cells.The apoptosis rate was (53.26 ± 1.78)% in the black garlic high-dose group,and (3.68 ±0.11)% in the control group.Black garlic extract affected cell cycle,upregulated bax expression,and downregulated bcl-2 expression.Conclusions Black garlic extract significantly induced the apoptosis of HeLa cells.This effect might be caused through increasing G2/M cells or changing expressions of bax and bcl-2.