Let-7a is involved in the pathogenesis of chronic eosinophilic rhinosinusitis with nasal polyp by regulating BAFF

VernacularTitle:let-7a通过调控BAFF参与嗜酸粒细胞型CRSwNP发病机制的初步研究
Author: Xinhao ZHANG ; Nan WANG ; Qimiao FENG ; Xiaobo LONG
Publication Type:Journal Article
Keywords: MicroRNAs/PD/ME; Membrane proteins/PD/ME; Nasal polyps/CO/DT/ME; Sinusitis/CO/DT/ME
From: Journal of Chinese Physician 2015;17(8):1160-1163
CountryChina
Language:Chinese
Abstract: Objective To explore the regulatory effect of miRNA on B-cell activating factor (BAFF) in chronic eosinophilic rhinosinusitis with nasal polyp (Eso CRSwNP).Methods Ten nasal mucosal samples were collected from Eso CRSwNP patients who were admitted and treated in our hospital between January 2012 and February 2013.Normal nasal mucosal tissues (n =10) served as control.The miRNA-targeting BAFF was predicted by bioinformatics tools.Immunohistochemistry and real time polymerase chain reaction (RT-PCR) were used to assess the protein expression of BAFF and the predicted miR-NA.The correlation between the predicted miRNA and BAFF was analyzed.Results Down-regulated expression of let-a was confirmed in Eos CRSwNP,while the BAFF protein expression was increased.Let-a was positively correlated with BAFF in nasal epithelia.Conclusions Let-a might contribute to mucosal eosinophilia in eosinophilic CRSwNP via targeting BAFF.