Diagnostic utility of WT-1, AR and Ki-67 in Sertoli cells and germ cells for testicular biopsy
10.13315/j.cnki.cjcep.2015.08.002
- VernacularTitle:WT-1、AR和Ki-67在睾丸穿刺活检组织病理诊断中的应用
- Author:
Hui WU
;
Yuhong MENG
;
Ping LU
;
Haoyong NING
;
Liu HONG
;
Xiaoling KANG
;
Mingang DUAN
- Publication Type:Journal Article
- Keywords:
testicular biopsy;
Sertoli cell;
WT-1;
AR;
Ki-67
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(8):846-849
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To explore the specific diagnostic markers of Sertoli cells and germ cells for testicular biopsy. Methods Normal testis from 3 patients who suffered from carcinoma of the prostate and treated with testis castration, and 15 testicular biopsy tissues were stained by EnVision two steps with WT-1, AR and Ki-67. Results The expression of WT-1 and AR protein were found in Sertoli cells from the 3 normal testis and 15 testis biopsy, the positive rate were all 100%, and non was positive in germ cells. The germ cell was positive for Ki-67, and the positive rate was 100%. Conclusions WT-1, AR and Ki-67 may be the specific diagnostic markers of Sertoli cells and germ cells for testicular biopsy, which may contribute to the diagnosis of testicular biopsy.
