Role of SOCS3 in tubulointerstitial injury of mice with diabetes
10.13315/j.cnki.cjcep.2015.08.014
- VernacularTitle:SOCS3在糖尿病小鼠肾小管间质病变中的作用
- Author:
Xiang GAO
;
Lingling XING
;
Shuxia LIU
;
Yunxia DU
;
Kejun LI
;
Siyuan LIU
;
Qingjuan LIU
- Publication Type:Journal Article
- Keywords:
diabetic nephropathy;
tubular epithelial cell;
SOCS3;
STAT3
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(8):900-903
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the role of SOCS3 on diabetic renal injury. Methods Male CD-1 mice were randomly divided into four groups:control group, diabetic group, empty plasmid vector transfection group and SOCS3 plasmid transfection group. The diabet-ic mice were induced by intraperitoneal injection of STZ at a dose of 150 mg/kg body weight. The mice of transfection group were re-ceived an injection of SOCS3 plasmid or empty vector at every 7 days thereafter. Specimens were collected at 12 week after STZ injec-tion. The morphological changes of tubular epithelial cells were observed by transmission electron microscope. RT-PCR and immuno-histochemistry were used to determine the mRNA and protein expression of CK18 and α-SMA. Western blotting analysis was used to determine the protein expression of SOCS3, p-STAT3, CK18 and α-SMA. Results SOCS3 overexpression in kidney down-regulated the levels of p-STAT3 andα-SMA but up-regulated the expression of CK18. Conclusion Overexpression of SOCS3 can ameliorate the tubular epithelial-mesenchymal transdifferentiation of diabetic mice via inhibiting the phosphorylation of STAT3.
