Preparation and in vitro release behavior of adiponectin microsphere-scaffold composite
10.3969/j.issn.1001-3733.2015.05.004
- VernacularTitle:脂联素微球复合 PLGA/β-TCP 支架材料的制备及生物活性评价
- Author:
Yuwei WU
;
Hui LU
;
Hongcheng HU
;
Dan LI
;
Yuan GUO
;
Zichen LI
;
Zhihui TANG
- Publication Type:Journal Article
- Keywords:
Chitosan;
Microsphere;
PLGA;
β-TCP;
Adiponectin(APN)
- From:
Journal of Practical Stomatology
2015;(5):615-618
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a bone substitute using microsphere scaffold containing adiponectin(APN)and to investigate the release behavior of the scaffold in vitro.Methods:Chitosan microsphere was developed by an emulsion-ionic cross-linking method. Poly (L-lactic-co-glycolic)acid (PLGA)and β-tricalcium phosphate (β-TCP)were used to prepare microsphere scaffold containing APN.The morphology,particle size,drug loading,incorporation efficiency and release behavior of the microsphere were examined. Results:The APN containing microsphere showed good spherical geometry,suitable size and microporosity under scanning electron microscope.The average diameter of the milipore was 20 -200 μm;the drug loading and incorporation efficiency were 1 .3% and 70.3% respectively.The controled-release process continued for 91 days.The extract solution from the APN microsphere-scaffold promoted MC3T3 cell proliferation without cytotoxicity.Conclusion:The APN microsphere-scaffold has sustained release function and may promote osteoblast proliferation.
