The effect of IQGAP1 on the proliferation and invasion of oral squamous cell cancer cells
10.3969/j.issn.1001-3733.2015.05.028
- VernacularTitle:IQGAP1对口腔鳞状细胞癌细胞增殖与侵袭的影响
- Author:
Xin LIU
;
Xin XIE
- Publication Type:Journal Article
- Keywords:
IQGAP1;
Oral squamous cell cancer(OSCC);
Proliferation;
Invasion
- From:
Journal of Practical Stomatology
2015;(5):716-719
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of IQ motif containing GTPase activating protein 1 (IQGAP1 )in oral squamous cell cancer(OSCC)tissue,and to explore the effects of IQGAP1 on cell proliferation and invasion as well as its underlying mechanism. Methods:Expression levels of IQGAP1 in tumor and adjacent normal tissues were examined by western blot and RT-PCR.OSCC cell line SCC-4 cells was transfected with the recombinant plasmid-pcDNA3.1 -IQGAP1 by lipofectamine,and then treated with an Akt in-hibitor.The phosphorylation of Akt,cell proliferation and invasion were detected by western blot,MTT assay and Transwell invasion as-say respectively.Results:Protein and mRNA expression levels of IQGAP1 were higher in cancer tissue than in adjacent normal tissue (P <0.05).Transfection of pcDNA3.1 -IQGAP1 increased IQGAP1 expression,enhanced the capability of cell proliferation and inva-sion (P <0.05),increased p Akt level in the cells.Preconditioning with an Akt inhibitor reduced p Akt level.Furthermore,silencing Akt pathway blocked the increase of cell proliferation and invasion induced by IQGAP1 overexpression(P <0.05).Conclusion:IQ-GAP1 overexprission can mediate the ability of proliferation and invasion of OSCC cells by regulating the activation of Akt pathway.
