Role of PI3K/Akt signal pathway in BMSCs migration induced by serum of rats with severely burn
10.3969/j.issn.1671-8348.2015.27.003
- VernacularTitle:PI3K/Akt 信号通路在严重烧伤大鼠血清诱导 BMSCs 迁移中的作用
- Author:
Maohua LI
;
Miao TENG
;
Lei GUO
- Publication Type:Journal Article
- Keywords:
burns;
mesenchymal stem cells;
microtubules;
migration;
PI3K/Akt;
signaling pathway
- From:
Chongqing Medicine
2015;(27):3752-3755
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of severely burned rats serum on migration of BMSCs and mechanism.Methods Severely burned rats model was established,and the preparation of severely burned rats serum.Experimental groups:normal train-ing group(containing 10% fetal bovine serum,group C),burn serum group(containing 10% burns in the rat serum,group B),burn serum+blockers(10% burns in the rat serum+final concentration of 10 μmol/L PI3K signaling pathway inhibitor LY294002 train-ing,group B+LY).Activity of cells was examined with MTT;migration of cells was examined with Transwell chambers testing;protein expression of p-AKT/AKT was determined with Western blot;microtubule structure of cells was examined with immuno-fluorescence.Results Compared with group C,group B burn serum treatment after 24 h,BMSCs activity(P <0.01),p-AKT levels (P <0.05),increased migration quantity(P <0.001);cell microtubule structures appear rupture,after adding inhibitor,compared with group B,group B+LY BMSCs activity(P <0.01),to reduce the number of migration(P <0.001),p-lower AKT(P <0.05), cell microtubule structure similar to the normal group.Conclusion Severely burned rats serum can promote BMSCs migration,may burn serum cytokine activation of PI3K/AKT signal pathway,resulting in cell microtubule structure change,promote the migration of BMSCs.
