Establishment of the endometriosis rat model in intimal window period
10.3969/j.issn.1671-8348.2015.27.019
- VernacularTitle:子宫内膜异位症大鼠内膜窗口期模型的建立?
- Author:
Fang CAO
;
Xiaoyang HUANG
;
Sumei GE
;
Ke XU
;
Li CHEN
- Publication Type:Journal Article
- Keywords:
endometriosis;
model,animal;
rat
- From:
Chongqing Medicine
2015;(27):3805-3807
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the endometrial receptivity by established the endometriosis rat model in intimal window period.Methods The autologous endometrial tissues of 22 sexually mature SD rats were transplanted into the homonymy abdomi-nal wall and offside uterine mesometrium.After four weeks,we randomly dissected two rats and observed the growth of etopic en-dometrium.The EMs rats were mated with male rats in the same cage and vaginal plug was observed in the next day.The histomor-phology of eutopic and ectopic endometrial was observed in the fifth day of pregnancy.Results Ectopic endometrium was grown in the abdominal wall and mesometrium,which formed a small bulge cystic mass in 20 rats.All foci was showed adhesion to the sur-rounding tissues in a different extent.In the light microscope,the morphology of ectopic endometrial was as the same as nomal en-dometrium.The establishment of the endometriosis rat mode is successful.Vaginal plug was positive the next day.Nonoperative side uterine was full of fluid in the fifth day of pregnancy.Eutopic and ectopic endometrium were in the secretory phase..Conclusion It is a successful method to establish a rat model of endometriosis by autotransplantion of endometrium.The endometrium of EMs model rats is in embryo implantation window period,and it provides a convenient model for studying endometrial receptivity.
