Improved tissue piece inoculation and identification of mouse vascular smooth muscle cell
10.3969/j.issn.1671-8348.2015.25.005
- VernacularTitle:改良组织块贴壁法培养小鼠主动脉血管平滑肌细胞及生物学鉴定?
- Author:
Lin HE
;
Tao LIU
;
Haoyu WANG
;
Lirong REN
- Publication Type:Journal Article
- Keywords:
vascular smooth muscle cells;
primary culture;
tissue piece;
mouse
- From:
Chongqing Medicine
2015;(25):3470-3472
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the method of primary culture and biological characteristics of aortic vascular smooth mus-cle cells (VSMC)in mice,providing experimental material for cellular and molecular scientific research of vascular disease. Methods Thoracic and abdominal aortas in mice were isolated and VSMC were obtained by using improved method of tissue piece inoculation.Digested with trypsin and passaged,VSMC were purified with differential adherence method.The conditions of cellular morphology and growth were observed under inverted phase contrast microscope,and VSMC were identified with hematoxylin-eosin (HE)staining and immunofluorescence.Results VSMC were isolated successfully and grown vigorously with good bioactivity,the cells had a radial or typicalpeak-valleylike growth,and showed fusiform,abundant cytoplasm with large and round or oval nucle-us by HE staining,the expressions of specific cytoplasmicα-smooth muscle actin were positive by immunofluorescence stain.Conclu-sion It can isolate and cultivate VSMC with high purity and good activity under in vitro conditions with simple,economical,reliable method.
