The effect of knockdown A20 expression on the proliferation, apoptosis and migration of MCF-7 cells
- VernacularTitle:RNA干扰A20基因的表达对人乳腺癌细胞MCF-7增殖、凋亡和迁移的影响
- Author:
Mingjie YU
;
Yuanhong XU
;
Ping WANG
- Publication Type:Journal Article
- Keywords:
A20 gene;
human breast cancer cell line MCF-7;
RNA interference;
cell proliferation;
cell apopto-sis;
cell migration.
- From:
Acta Universitatis Medicinalis Anhui
2015;50(9):1215-1219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of knockdown A20 expression on the proliferation, apoptosis and migra-tion of MCF-7 cells and to evaluate the potential value of the A20 gene as the therapeutic target of breast cancer. Methods Synthesized siRNA targeted to A20 gene or negative control siRNA were transfected into MCF-7 cells by using lipofectamine 2000. CCK8 assay, Annexin V and 7-AAD double staining cytometry, Transwell assay were performed to investigate the effect of knockdown A20 mRNA expression on the proliferation, apoptosis, migration of MCF-7 cells, respectively. Results It can inhibit the proliferation and migration as well as promote the apoptosis in MCF-7 cells by knockdown A20 mRNA expression. Conclusion A20 gene plays an important role in the prolif-eration, apoptosis and migration of MCF-7 cells and it could be a potential therapeutic target of breast cancer.
