The application of alpha-fetoprotein isoform L2 test in screening Down syndrome
10.3969/j.issn.1673-4130.2015.18.011
- VernacularTitle:甲胎蛋白变异体L2在唐氏综合征筛查中的应用
- Author:
Jing YU
;
Limei HE
;
Heyu HU
;
Li ZHANG
;
Fuhua CHEN
;
Bo GONG
- Publication Type:Journal Article
- Keywords:
Down syndrome;
time-resolved fluorescence;
alpha-fetoprotein;
alpha-fetoprotein isoform
- From:
International Journal of Laboratory Medicine
2015;(18):2643-2644
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore significance of alpha‐fetoprotein isoform L2(AFP‐L2) in the screening of Down syndrome in pregnant women ,so as to provide references for clinical application .Methods A total of 250 healthy pregnant women and 22 preg‐nant women with Down syndrome were enrolled in this study .Serum specimens were collected and AFP‐12 was separated and cap‐tured by using the magnetic bal ,time‐resolved fluorescence immunoassay was used to detect levels of AFP and AFP‐L2 ,and the percentage of AFP‐L2 (AFP‐L2% ) was calculated .Results The serum level of AFP of pregnant women with Down syndrome [(20.2±4.2)ng/mL]was lower than that of healthy pregnant women[(46.7±19.9)ng/mL],and had statistically significant difference(P<0 .05) .Serum AFP‐L2% of pregnant women with Down syndrome was higher than that of healthy pregnant women , and had statistically significant difference(P<0 .05) .Conclusion Detection of AFP level and AFP‐L2% could be an indicator for Dow n syndrome screening .
