Cloning,Expression and Diagnostic Application of Different Fragments of Human Carboxypeptidase
10.3969/j.issn.1671-7414.2015.04.003
- VernacularTitle:人羧基肽酶 H不同区段抗原的克隆表达及其初步应用
- Author:
Jiwen HU
;
Xiqin YANG
;
Keliang ZONG
;
Lanqin GUO
;
Xiaoguo SONG
;
Guohua WANG
;
Ximing LIU
;
Cuixia ZHU
;
Yanfeng ZHAO
;
Xiaoyan FENG
;
Heqiu ZHANG
- Publication Type:Journal Article
- Keywords:
human carboxypeptidase H;
autoantibody;
newly diagnosed type 2 diabetes;
latent autoimmune diabetes in adults
- From:
Journal of Modern Laboratory Medicine
2015;(4):10-13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain different fragments of human carboxypeptidase H,and evaluate the diagnostic application of the recombination carboxypeptidase H in detecting autoantibody.Methods The coding gene of carboxypeptidase H was ob-tained by RT-PCR.The corresponding prokaryotic expression vectors were constructed and transformed into E.coli to in-duce the expression of the recombination different fragments of carboxypeptidase H.Using these antigen fragments as the coating antigens,the enzyme-linked immunosorbent assay (ELISA)was established for the detection of carboxypeptidase H autoantibody in 95 newly diagnosed type 2 diabetes patients.Results Three fragments of human carboxypeptidase H were obtained,in which the 42~476aa fragment antigen was ideal one.Using the full-length carboxypeptidase H as coating anti-gen,the positive rate of carboxypeptidase H autoantibody was 8.42%.Conclusion Because of the favorable antigenicity,the 42~476aa fragment antigen of carboxypeptidase H could be the candidate antigen for discrimination and diagnosis of latent autoimmune diabetes in adults.
