Clinical Applification of Combined Detection of Pencilliosis Marneffei in a Non-HIV-infected Child
10.3969/j.issn.1671-7414.2015.04.024
- VernacularTitle:三种方法联合检测在非 HIV 感染儿童马尔尼菲青霉病的临床应用
- Author:
Weina HE
;
Danhong SU
;
Dongyue LU
;
Hua DING
;
Jianjiang HUA
;
Yue HE
;
Hong LIANG
- Publication Type:Journal Article
- Keywords:
non-HIV-infection;
child;
pencilliosis marneffei;
combined detection
- From:
Journal of Modern Laboratory Medicine
2015;(4):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the diagnostic value of pencilliosis marneffei (PM)in a non-HIV-infected child with the com-bined detection of aspergillosis galactomannan,fungus Glucan(1-3)-β-D and boold culuture.Methods The venous blood specimen from the child was collected for the quantified detection of aspergillosis galactomannan,fungus Glucan(1-3)-β-D. The growth and colonial morphology of fungus was inspected with the positive blood culture and the characteristics of fun-gus smear were observed under microscope.Results The result of aspergillosis galactomannan was 14.45 μg/L and fungus Glucan (1-3)-β-D 77.14 pg/ml.Penicillium marnrffei was identified using blood culture.It was mycelia form under 25℃ and the salouraud medium produced water soluble claret-red pigment produced.It was mycelia form under 35℃ and the colony was gyri creases,the characteristic broom-like hypha and separation hypha could be found under microscope.Conclusion It is effective for the early diagnosis and therapy of PM with the combination detection of aspergillosis galactomannan,fungus Glucan (1-3)-β-D and boold culuture and have better clinical diagnosis value.
