Prevalence of Major Methicillin-Resistant Staphylococcus aureus Clones in Korea Between 2001 and 2008.
10.3343/alm.2016.36.6.536
- Author:
Gi Su KANG
1
;
Yung Hee JUNG
;
Hwa Su KIM
;
Yeong Seon LEE
;
Chan PARK
;
Kwang Jun LEE
;
Jeong Ok CHA
Author Information
1. Division of Antimicrobial Resistance, Center for Infectious Disease, National Research Institute of Health, Centers for Disease Control and Prevention (KCDC), Cheongju, Korea. jeongok22@gmail.com
- Publication Type:Original Article
- Keywords:
Korean MRSA clones;
Multilocus sequence typing;
DiversiLab;
Genetic diversity
- MeSH:
Bacterial Proteins/genetics;
DNA, Bacterial/genetics/metabolism;
Electrophoresis, Gel, Pulsed-Field;
Hospitals;
Humans;
Methicillin-Resistant Staphylococcus aureus/*genetics/isolation & purification;
Multilocus Sequence Typing;
Multiplex Polymerase Chain Reaction;
Prevalence;
Republic of Korea/epidemiology;
Staphylococcal Infections/diagnosis/*epidemiology/microbiology
- From:Annals of Laboratory Medicine
2016;36(6):536-541
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) are important pathogens causing nosocomial infections in Korean hospitals. This study aimed to investigate the epidemiological and genetic diversity of clinical S. aureus isolates in healthcare settings from 2001 to 2008. METHODS: Samples and data were obtained from 986 individuals as part of the National Antimicrobial Surveillance Project, involving 10 regions nationwide. Molecular typing studies, including multilocus sequence typing (MLST) and staphylococcal cassette chromosome mec (SCCmec) typing were performed, and a representative clone of Korean MRSA was classified by combinational grouping using a DiversiLab (DL; bioMérieux, France) repetitive element polymerase chain reaction (rep-PCR) system. RESULTS: Nine Korean MRSA clones (KMRSA-1 to -9) were identified by analysis of genetic backgrounds and molecular characteristics. KMRSA-1 to -3, expressing clonal complex (CC) 5 (carrying SCCmec II), CC8 (carrying SCCmec III), and CC72 (carrying SCCmec IV) were spread nationwide. In contrast, KMRSA-6 was highly prevalent in Gyeongsangnam-do, and KMRSA-4 was highly prevalent in Jeollanam-do and Jeollabuk-do. CONCLUSIONS: Epidemic KMRSA clones were genetically similar to major clones identified from the USA, with the exception of KMRSA-2, which had the SCCmec III type. Our results provide important insights into the distribution and molecular genetics of MRSA strains in Korea and may aid in the monitoring of MRSA spread throughout the country.
