Reality of Negative anti-HBc and Positive anti-HBe Results of Testing HBV-M with ELISA
10.3969/j.issn.1671-7414.2015.04.028
- VernacularTitle:ELISA 法检测 HBV-M 结果呈抗 HBc（－）抗 HBe（＋）的科学评价
- Author:
Zhe AN
;
Sipeng LI
;
Wei DONG
;
Ni ZHANG
;
Miaoxian LI
;
Xiangling WANG
- Publication Type:Journal Article
- Keywords:
anti-HBe;
anti-HBc;
enzyme-linked immunosorbent assay(ELISA);
chemiluminecence microparticle immunoassay (CMIA)
- From:
Journal of Modern Laboratory Medicine
2015;(4):99-101
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the reality of ELISA testing results with negative anti-HBc and positive antiHBe.Methods CMIA was carried out to retest antiHBc and antiHBe of 105 samples which were initially tested to have negative antiHBc and positive antiHBe.Results Among the 105 samples retested by CMIA,there were three different results,positive antiH-Bc with positive antiHBe,negative antiHBc with negative antiHBe and positive antiHBc with negative antiHBe,whose pro-portions were 72.38%,21.91% and 5.71% respectively;the fasle negative rates of antiHBc were 78.10% in total and 93.33%,96.15% and 47.37% in 3 subgroup with S/CO 1.00~ 1.20,1.20~2.00 and 2.00~ 3.00,respectively;the true positive rates of antiHBe were 72.38% in total and 42.86%,88.14% and 56.25% in 3 subgroups with S/CO 0.00~0.10, 0.10~0.80 and 0.80 ~ 1.00,respectively.Conclusion HBV-M results with negative antiHBc and positive antiHBe by ELISA could give suggestive value and not reflect true information about antiHBc and antiHBe with three alternatives which would be obtained through retesting by a second assay.
