Evaluation and Disposal of the Carryover between the Chemistry and Immunoassay in Beckman Coulter Laboratory Automation System
10.3969/j.issn.1671-7414.2015.04.037
- VernacularTitle:Beckman Coulter 实验室自动化系统生化免疫样品间携带污染评估与解除
- Author:
Yong XIA
;
Ling JI
;
Anping XU
;
Song LING
;
Hao XUE
;
Ruoyang ZHENG
- Publication Type:Journal Article
- Keywords:
laboratory automation system;
sample to sample;
carryover
- From:
Journal of Modern Laboratory Medicine
2015;(4):125-127
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the Carryover between the chemistry and immunoassay in Beckman Coulter Laboratory Au-tomation System and decide to whether sharing samples for testing between chemistry and immunoassay systems or not. Methods According to a certain order,high concentration samples and low concentration samples of HCG with different sample volume (500 μl,2 000 μl)were tested on Beckman AU5421 automatic biochemical analyzer.The HCG of low concen-tration samples were then tested to evaluate the carryover between the chemistry and immunoassay and explored the correc-tive procedure to deal with the carryover by increasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.Results Under different sample volume,the carryover in a single module and as a whole of the beckman AU5421 automatic biochemistry analyzer were 5.44,15.47,23.51 and 45.96 ppm respectively (t=14.553,P <0.001;t=5.527,P =0.005;t=3.985,P =0.016;t=20.457,P <0.001).By increasing special cleaning process the carryover of 0.22 ppm was detected in 500 μl sample volume of the beckman AU5421 automatic biochemistry analyzer as a whole.Conclusion The car-ryover between the chemistry and immunoassay in Beckman Coulter Laboratory Automation System could been sovled by in-creasing special cleaning process of beckman AU5421 automatic biochemistry analyzer.
