Exploration of Appropriate Cross-Matching Methods for Positive Patients of the Direct Antiglobulin Test
10.3969/j.issn.1671-7414.2015.04.039
- VernacularTitle:直接抗人球蛋白试验阳性患者交叉配血方法探讨
- Author:
Jiangang LI
;
Jianing ZHAO
;
Hua XU
;
Suoliang YAO
- Publication Type:Journal Article
- Keywords:
direct antiglobulin test;
cross-matching;
washed red blood cell
- From:
Journal of Modern Laboratory Medicine
2015;(4):130-132
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the appropriate cross-matching methods for the direct antiglobulin test (DAT)positive patients.Methods The DAT was used to screen the irregular antibodies on the surface of erythrocytes.The microcolumn gel test was used for blood identification.The antiglobulin test and manual polybrene test were used for cross-matching.Re-sults A total of five DAT positive cases were collected in study.The blood groups of each case were detected,including A-DCcEe,O-DCCee,O-DCcee,A-DCCee and A-DCcee.The donor samples with corresponding blood-antigen negative were cho-sen for cross-matching,and the agglutination intensity of each cross-matching tube was weaker than the control.The transfu-sion of washed red blood cells was suggested.Finally,each transfusion was safe and efficient.Conclusion The infusion of the washed red blood cells with corresponding blood-antigen negative could efficiently ensure the safety of blood transfusion.
